STEM CELL EXPANSION
Stem Cells Expansion using Cytodex 3 microcarriers in 50ml Gas Membrane Chamber. Live/Dead staining images of Human Fetal Mesenchymal Stem Cells (HFMSCs) grown on Cytodex 3 microcarriers over 7 days culture period in TisXell Batch System. Green indicates live cells and Red indicates dead cells.
QUANTITATIVE ANALYSIS of VASCULARIZATION
One month after transplantation, more blood vessels can be seen clustering around the TEBG scaffolds (Group I) than the acellular scaffolds (Group II). Micro CT image of the vasculature in the defect area showed that TEBG (Group I) induced an intense network of new blood vessels infiltrating the defect area in contrast to the limited vascular penetration where acellular scaffolds (Group II) had been transplanted (Dotted red circle indicates location of transplanted scaffold, threshold¼ 190); (B) Quantitative analysis of vascularization by micro CT showed that TEBG group (Group I) achieved higher vascular volume than control group (Group II)
COMPARISON OF BIOREACTORS
The comparative data showed that TisXell regeneration system outperformed others in In vitro where it was seen to have Faster cell proliferation, More cellularity, More homogenous neo-tissue distribution, More robust osteogenesis and In vivo More ectopic bone formation as well as Higher human murine chimerism rate
MICROCARRIERS in Spinner Flask vs TisXell
Comparison studies between Spinner Flask and TisXell System of Stem Cells Expansion using Cytodex 3 microcarriers. Live/Dead staining images of Human Fetal Mesenchymal Stem Cells (HFMSCs) grown on Cytodex 3 microcarriers over 7 days culture period in Spinner Flask (Left) and TisXell System (Right). Green indicates live cells and Red indicates dead cells.