APPLICATIONS

TisXell Applications

3D Culture on Decellularised Heart Matrix

Live/Dead staining images of Human Fetal Mesenchymal Stem Cells (HFMSCs) grown on decellularised mice whole heart matrix over 7 days culture period in TisXell System. Green indicates live cells and Red indicates dead cells.

SPHEROID FORMATION in Static vs TisXell

Light Microscopy images of Spheroids using Prostate Cancer Cells (PC3) over 4 days culture period under static conditions in ultra-low adherent 24- well plate (Left) and TisXell Batch System (Right).

FORMATION OF SPHEROIDS ON PC3

Light Microscopy images of Spheroids using Prostate Cancer Cells (PC3) over 4 days culture period in TisXell Batch System.

FORMATION OF SPHEROIDS (Confocal)

Live/Dead staining images of Spheroids using Human Fetal Mesenchymal Stem Cells (HFMSCs) over 5 days culture period in TisXell System. Green indicates live cells and Red indicates dead cells.

FORMATION OF SPHEROIDS ON HFMSCs

Light Microscopy images of Spheroids using Human Fetal Mesenchymal Stem Cells (HFMSCs) over 48 hours culture period in TisXell System.

STEM CELL EXPANSION

Stem Cells Expansion using Cytodex 3 microcarriers in 50ml Gas Membrane Chamber. Live/Dead staining images of Human Fetal Mesenchymal Stem Cells (HFMSCs) grown on Cytodex 3 microcarriers over 7 days culture period in TisXell Batch System. Green indicates live cells and Red indicates dead cells.

3D CULTURE ON POLYACTIVE SCAFFOLDS

Live/Dead staining images of Human Fetal Mesenchymal Stem Cells (HFMSCs) grown on Polyactive Scaffolds over 3 days static culture period in 24-well plate followed by 6 days culture period in TisXell System. Green indicates live cells and Red indicates dead cells.

MICROCARRIER RUN on TisXell

Stem Cells Expansion using Cytodex 3 microcarriers. Live/Dead staining images of Human Fetal Mesenchymal Stem Cells (HFMSCs) grown on Cytodex 3 microcarriers over 7 days culture period in TisXell System. Green indicates live cells and Red indicates dead cells.

QUANTITATIVE ANALYSIS of VASCULARIZATION

One month after transplantation, more blood vessels can be seen clustering around the TEBG scaffolds (Group I) than the acellular scaffolds (Group II). Micro CT image of the vasculature in the defect area showed that TEBG (Group I) induced an intense network of new blood vessels infiltrating the defect area in contrast to the limited vascular penetration where acellular scaffolds (Group II) had been transplanted (Dotted red circle indicates location of transplanted scaffold, threshold¼ 190); (B) Quantitative analysis of vascularization by micro CT showed that TEBG group (Group I) achieved higher vascular volume than control group (Group II)

COMPARISON OF BIOREACTORS

The comparative data showed that TisXell regeneration system outperformed others in In vitro where it was seen to have Faster cell proliferation, More cellularity, More homogenous neo-tissue distribution, More robust osteogenesis and In vivo More ectopic bone formation as well as Higher human murine chimerism rate

MICROCARRIERS in Spinner Flask vs TisXell

Comparison studies between Spinner Flask and TisXell System of Stem Cells Expansion using Cytodex 3 microcarriers. Live/Dead staining images of Human Fetal Mesenchymal Stem Cells (HFMSCs) grown on Cytodex 3 microcarriers over 7 days culture period in Spinner Flask (Left) and TisXell System (Right). Green indicates live cells and Red indicates dead cells.

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